Press release: US scientists find 15 million-year-old…

5 February 2015

US scientists find 15 million-year-old mollusk protein

Ecphora_175

A 15-million year old fossil gastropod, Ecphora, from the Calvert Cliffs of southern Maryland is depicted. The golden brown color arises from the original shell-binding proteins and pigments preserved in the mineralized shell. Photo courtesy of John Nance.

Washington, D.C.— A team of US scientists have found “beautifully preserved” 15 million-year-old thin protein sheets in fossil shells from southern Maryland. Their findings are published in the inaugural issue of Geochemical Perspectives Letters, the new peer-reviewed journal of the European Association of Geochemistry.

The team – John Nance, John Armstrong, George Cody, Marilyn Fogel, and Robert Hazen – from the Carnegie Institution (Washington DC) collected samples from Calvert Cliffs, along the shoreline of the Chesapeake Bay, a popular fossil collecting area. The Cliffs were named after the mother of the English explorer John Smith (of John Smith and Pocohontas fame). The team found fossilized shells of a snail-like coastal-dwelling mollusk called Ecphora that lived in the mid-Miocene era-between 8 and 18 million years ago.

Ecphora is known for an unusual reddish-brown shell color, making it one of the most distinctive North American mollusks of its era. This coloration is preserved in fossilized remains, unlike the fossilized shells of many other fossilized mollusks from the Calvert Cliffs region, which have turned chalky white over the millions of years since they housed living creatures.

Shells are made from crystalline compounds of calcium carbonate interleaved with an organic matrix of proteins and sugars proteins and sugars. These proteins are called shell-binding proteins by scientists, because they help hold the components of the shell together. They also contain pigments, such as those responsible for the reddish-brown appearance of the Ecphora shell. These pigments can bind to proteins to form a pigment-protein complex.

The fact that the coloration of fossilized Ecphora shells is so well preserved suggested to the research team that shell proteins bound to these pigments in a complex might also be preserved. “We were amazed to find that the shells, once dissolved in dilute acid, released intact thin sheets of shell proteins more than a centimeter across” said main author John Nance. Chemical analysis including spectroscopy and electron microscopy of these sheets revealed that they are indeed shell proteins that were preserved for up to 15 million years.

“These are some of the oldest and best-preserved examples of a protein ever observed in a fossil shell,” said author Robert Hazen.

Remarkably, the proteins share characteristics with modern mollusk shell proteins. They both produce thin, flexible sheets of residue that’s the same color as the original shell after being dissolved in acid. Of the 11 amino acids found in the resulting residue, aspartate and glutamate are prominent, which is typical of modern shell proteins. Further study of these proteins could be used for genetic analysis to trace the evolution of mollusks through the ages, as well as potentially to learn about the ecology of the Chesapeake Bay during the era in which Ecphora thrived.

Commenting, the journal’s founding co-editor Bruce Watson (Rensselaer Polytechnic Institute, USA) said:

“As the authors say, this find presents a potentially exciting opportunity. It gives us a window back around 15 million years, which is about the same time the first mammoths appeared. This gives us an unprecedented possibility for amino acid sequencing and phylogenetic analysis of gastropod evolution. It is remarkable to be able to confirm that these ancient proteins share many of the characteristics of modern mollusk proteins”.

The European Association of Geochemistry President, Liane G. Benning (University of Leeds, UK), added:

“We are happy to launch our new Society Journal with such a high quality paper. The team described some of the oldest fossil samples of this type ever found. Ecphora is now an extinct species, but this work will help us understand the relationship between these beautiful creatures and other mollusks of the period”.

ENDS

Notes for Editors

Contact Details
• EAG Press Office (also for copies of paper) Tom Parkhill, tom@parkhill.it, tel: +39 349 238 8191
• John Nance (lead author) nancejr@co.cal.md.us
• Bruce Watson (Journal Co-Editor) watsoe@rpi.edu
• Liane G. Benning (EAG President) L.G.Benning@leeds.ac.uk

This work was supported in part by the NASA Astrobiology Institute, the Deep Carbon Observatory, the Hazen Foundation, and the Carnegie Institution for Science. It is published in the first edition of the Geochemical Perspectives Letters, a new international peer-reviewed journal that publishes high-quality articles spanning the geochemical sciences. It is published by the European Association of Geochemistry, which is Europe’s leading geochemistry organization. See websites http://www.geochemicalperspectivesletters.org/ and http://www.eag.eu.com/

Full reference: Nance, J.R., Armstrong, J.T, Cody, G.D., Fogel, M.L., Hazen, R.M. (2015) Preserved macroscopic polymeric sheets of shell-binding protein in the Middle Miocene (8 to 18 Ma) gastropod Ecphora. Geochem. Persp. Let. 1, 1-9, available for download after embargo at: http://www.geochemicalperspectivesletters.org/article1501

See also the Carnegie Institution for Science (carnegiescience.edu), a private, nonprofit organization headquartered in Washington, D.C., with six research departments throughout the U.S.

The 2015 Goldschmidt Conference, which is the World’s leading geochemistry conference, will take place from 16-21 August 2015 in Prague (http://goldschmidt.info/2015/).

Press release on EurekAlert

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